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KMID : 0363620200410030055
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Journal of Korean Oriental Medicine
2020 Volume.41 No. 3 p.55 ~ p.65
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Bacterial Reverse Mutation Test of CP pharmacopunture
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Hwang Ji-Hye
Ku Ja-Seung
Jung Chul
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Abstract
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Objectives: This study aimed to evaluate the toxicity of CP pharmacopunture using bacterial reverse mutation test.
Methods: To determine the mutagenic potential of CP pharmacopunture, histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli (WP2uvrA, pKM101) strains were used. The negative (normal saline solution) and positive (Sodium azide, 2-Nitrofluorene, 2-Aminoanthracene, 9-Aminoacridine, and 4-Nitroquinoline N-oxide) control groups were used. To determine the dose levels of the main study, a dose range-finding study was conducted.
Results: As a results of the dose range-finding study, the growth inhibition by CP pharmacopunture was not evident at any dose levels in the absence and presence of metabolic activation. As a results of the main study, the mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the CP pharmacopuncture in the presence and absence of metabolic activation, showing no dose-related increase. In the positive control group, the number of revertant colonies was markedly increased by more than twice when compared to the negative control group.
Conclusion: According to the results of this study, CP pharmacopunture did not show any signs of mutagenic potential.
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KEYWORD
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CP pharmacopunture, capsaicin, toxicity, bacterial reverse mutation test, acupuncture
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